ACTIVE ACETYLCHOLINE-RECEPTOR FRAGMENT OBTAINED BY TRYPTIC DIGESTION OF ACETYLCHOLINE-RECEPTOR FROM TORPEDO CALIFORNICA

被引：29

作者：

BARTFELD, D

FUCHS, S

机构：

[1] Department of Chemical Immunology The Weizmann Institute of Science, Rehovot

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)90659-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tryptic digestion of acetylcholine receptor (AChR) from Torpedo californica did not change the pharmacological specificity and the pathological myasthenic acitivity of the receptor molecule. The product obtained after tryptic digestion was repurified by affinity chromatography on a toxin-Sepharose resin and was designated T-AChR. T-AChR has a sedimentation coefficient of 8.0S and in SDS acrylamide gel electrophoresis shows one major band with a molecular weight of 27,000. Immunological studies reveal that T-AChR binds to anti-AChR antibodies directed only against conformational antigenic determinants. © 1979.

引用

页码：512 / 519

页数：8

共 20 条

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