REORGANIZATION OF ACTIN IN PLATELETS STIMULATED BY THROMBIN AS MEASURED BY THE DNASE-I INHIBITION ASSAY

被引：220

作者：

CARLSSON, L

MARKEY, F

BLIKSTAD, I

PERSSON, T

LINDBERG, U

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 12期

关键词：

D O I：

10.1073/pnas.76.12.6376

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of thrombin stimulation on actin organization in human platelets has been analyzed by using the DNase I inhibition assay, which is selective for unpolymerized and filamentous actin. The results provide biochemical evidence for the suggestion that stimulation leads to rapid polymerization of actin. The measurements also reveal changes in the polymerization state of actin occurring after cell lysis. These changes are influenced by the concentration of free calcium in the extracts.

引用

页码：6376 / 6380

页数：5

共 42 条

[1] PHYSARUM ACTIN - OBSERVATIONS ON ITS PRESENCE, STABILITY, AND ASSEMBLY IN PLASMODIAL EXTRACTS AND DEVELOPMENT OF AN IMPROVED PURIFICATION PROCEDURE [J].