STEROL COMPOSITION OF TOBACCO CALLI SELECTED FOR RESISTANCE TO FENPROPIMORPH

被引：16

作者：

SCHALLER, H ^{[1
]}

MAILLOTVERNIER, P ^{[1
]}

BENVENISTE, P ^{[1
]}

BELLIARD, G ^{[1
]}

机构：

[1] UNIV PARIS 11,CNRS,URA 115,GENET & AMELIORAT PLANTES LAB,F-91405 ORSAY,FRANCE

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 08期

关键词：

NICOTIANA TOBACUM; SOLANACEAE; UV-MUTAGENESIS; PROTOPLASTS; RESISTANCE TO FENPROPIMORPH; ACCUMULATION OF 9-BETA; 19-CYCLOPROPYL STEROLS;

D O I：

10.1016/0031-9422(91)85097-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fenpropimorph, a fungicide used in agriculture, is a strong inhibitor of the cycloeucalenol-obtusifoliol isomerase in plants. Selection experiments, consisting of screening large populations of microcalli derived from UV-mutagenized protoplasts of tobacco for resistance to a lethal concentration (40 mg l-1) of fenpropimorph, resulted in the recovery of seven resistant calli. When grown in selective conditions, the resistant calli accumulated 9-beta,19-cyclopropyl sterols in higher amounts than control calli. After three consecutive passages on a selective medium, some calli may contain up to 99% of cyclopropyl sterols. These results suggest that some of the cyclopropyl sterols may be good surrogates of DELTA-5-sterols in the wild-type and resistant calli, and that phytotoxicity of fenpropimorph is not directly related to the inhibition of sterol biosynthesis in wild-type tobacco tissues.

引用

页码：2547 / 2554

页数：8

共 26 条

[1] STEROL BIOSYNTHESIS [J].