DEVELOPMENT OF A POLYMERASE CHAIN REACTION-PROBE TEST FOR IDENTIFICATION OF ALLOIOCOCCUS-OTITIS

被引：12

作者：

AGUIRRE, M ^{[1
]}

COLLINS, MD ^{[1
]}

机构：

[1] INST FOOD RES,AGR & FOOD RES COUNCIL,DEPT MICROBIOL,READING LAB,WHITEKNIGHTS RD,READING RG6 2EF,ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 08期

关键词：

D O I：

10.1128/JCM.30.8.2177-2180.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid polymerase chain reaction test was developed for specific identification of the human middle ear pathogen Alloiococcus otitis. Primers for the enzymatic amplification reaction were designed from highly specific sequences within the 16S rRNA gene. In addition, a confirmatory test based on hybridization of the polymerase chain reaction products to a specific internal probe was developed.

引用

页码：2177 / 2180

页数：4

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