NMR AND MOLECULAR MODELING INVESTIGATIONS OF THE NEUROPEPTIDE BRADYKININ IN 3 DIFFERENT SOLVENT SYSTEMS - DMSO, 9/1 DIOXANE/WATER, AND IN THE PRESENCE OF 7.4 MM LYSO PHOSPHATIDYLCHOLINE MICELLES

被引:62
作者
YOUNG, JK
HICKS, RP
机构
[1] Department of Chemistry, Mississippi State University, Mississippi
关键词
D O I
10.1002/bip.360340504
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The linear nonapeptide hormone bradykinin (Arg(1)-Pro(2)-pro(3)-Gly(4)-Phe(5)-Ser(6)-pro(7)-Phe(8) Arg(9)) is involved, either directly or indirectly, in a wide variety of physiological processes, particularly pain and hyperanalgesia. Additional evidence suggests that bradykinin also plays a major role in inflammatory response, asthma, sepsis, and symptoms associated with the rhinoviral infection. It has long been speculated that a beta-turn the C-terminus of bradykinin plays a major role in the biological activity of the neuropeptide. The beta-turn forming potential of bradykinin in three vastly different local chemical environments, DMSO, 9 : 1 dioxane/water, and in the presence of 7.4 mM lyse phosphatidylcholine micelles, was investigated using two-dimensional homonuclear nmr experiments coupled with simulated annealing calculations. The results of these investigations show that in all three systems residues 6-9 of the C-terminus adopt very similar beta-turn like structures. These results suggest that the beta-turn at the C-terminus of bradykinin is an important secondary structural feature for receptor recognition and binding. (C) 1994 John Wiley and Sons, Inc.
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页码:611 / 623
页数:13
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