GENERATION OF LYMPHOHEMATOPOIETIC CELLS FROM EMBRYONIC STEM-CELLS IN CULTURE

被引：643

作者：

NAKANO, T ^{[1
]}

KODAMA, H ^{[1
]}

HONJO, T ^{[1
]}

机构：

[1] OHU UNIV,SCH DENT,DEPT ANAT,FUKUSHIMA 963,JAPAN

来源：

SCIENCE | 1994年 / 265卷 / 5175期

关键词：

D O I：

10.1126/science.8066449

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An efficient system was developed that induced the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages by coculture with the stromal cell line OP9. This cell line does not express functional macrophage colony-stimulating factor (M-CSF). The presence of M-CSF had inhibitory effects on the differentiation of ES cells to blood cells other than macrophages. Embryoid body formation or addition of exogenous growth factors was not required, and differentiation was highly reproducible even after the selection of ES cells with the antibiotic G418. Combined with the ability to genetically manipulate ES cells, this system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.

引用

页码：1098 / 1101

页数：4

共 32 条

[1] MACROPHAGE LINEAGE SWITCHING OF MURINE EARLY PRE-B LYMPHOID-CELLS EXPRESSING TRANSDUCED FMS GENES [J].