PRINCIPLES AND METHODS FOR THE ANALYSIS AND PURIFICATION OF SYNTHETIC DEOXYRIBONUCLEOTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：37

作者：

WARREN, WJ

VELLA, G

机构：

[1] WATERS CORP,MILFORD,MA 01757

[2] BIOSEARCH,MILFORD,MA 01757

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 02期

关键词：

ANALYSIS OF SYNTHETIC DNA BY HPLC; PURIFICATION OF SYNTHETIC DNA BY HPLC; ANALYSIS AND PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES BY HPLC;

D O I：

10.1007/BF02921611

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The need for high-purity oligodeoxyribonucleotides for various applications has resulted in the development of novel synthesis, purification, and analytical techniques. A diversity of methods, including polyacrylamide slab gel electrophoresis, capillary gel electrophoresis, as well as high-performance liquid chromatography (HPLC), have been successfully used to aid in the characterization and isolation of these synthetic compounds. The information contained in this review article primarily details both the theoretical and practical aspects related to the use of HPLC for the analysis and purification of synthetic DNA. In addition, a variety of postsynthesis sample preparation protocols, commonly employed prior to and after HPLC, are described.

引用

页码：179 / 199

页数：21

共 24 条

[1] SEPARATION OF SYNTHETIC PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES FROM THEIR OXYGENATED (PHOSPHODIESTER) DEFECT SPECIES BY STRONG-ANION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].