SIZE DETERMINATION OF POLY(A) SEQUENCES IN BARLEY STRIPE MOSAIC-VIRUS RNA

被引：10

作者：

NEGRUK, VI ^{[1
]}

AGRANOVSKY, AA ^{[1
]}

SKRYABIN, KG ^{[1
]}

ATABEKOV, JG ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,DEPT VIROL,MOSCOW 117234,USSR

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80031-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The length of poly(A) fragments in barley stripe mosaic virus (BSMV) RNA was determined. Poly(A) fragments obtained from total BSMV RNA samples treated with a mixture of pancreatic RNase and T1 RNase were labeled at the 5′-end by [γ-32P]ATP and analyzed in 20% polyacrylamide gels containing 7 m urea. It was found that the length of BSMV RNA poly(A) fragments was 8 to 30 nucleotide residues, with 19 to 28 nucleotide residues fragments being predominant. © 1979 Academic Press, Inc.

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页码：450 / 453

页数：4

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