NEW PLANAR LIQUID-CHROMATOGRAPHIC TECHNIQUE - OVERPRESSURED THIN-LAYER CHROMATOGRAPHY

被引：204

作者：

TYIHAK, E ^{[1
]}

MINCSOVICS, E ^{[1
]}

KALASZ, H ^{[1
]}

机构：

[1] SEMMELWEIS UNIV MED,DEPT PHARMACOL,H-1082 BUDAPEST,HUNGARY

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 174卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)87038-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel planar liquid chromatographic technique using a pressurized ultramicro chamber (PUM chamber) has been developed. The sorbent layer is completely covered by a membrane under external pressure, so that the vapour phase above the layer is virtually eliminated. Solvent is admitted under pressure by means of a pump system. The main advantage of this technique, termed overpressured thin-layer chromatography (TLC), is the substantially shorter time required for separation than in classical column chromatography and TLC, with velocities of the mobile phase about as stable as in high-performance liquid chromatography, and aggressive reagents being applicable as in classical TLC. The method appears to be suitable for the accurate modelling of column chromatographic methods. © 1979.

引用

页码：75 / 81

页数：7