QUANTITATIVE IMMUNOGOLD LOCALIZATION OF DIPEPTIDYL PEPTIDASE-IV (DPP-IV) IN RAT-LIVER CELLS

被引：24

作者：

FUKUI, Y

YAMAMOTO, A

KYODEN, T

KATO, K

TASHIRO, Y

机构：

[1] KANSAI MED UNIV, DEPT PHYSIOL, MORIGUCHI, OSAKA 570, JAPAN

[2] KYUSHU UNIV, FAC PHARMACEUT SCI, HIGASHI KU, FUKUOKA 812, JAPAN

来源：

CELL STRUCTURE AND FUNCTION | 1990年 / 15卷 / 02期

关键词：

Basolateral surface; Bile canalicular surface; Dipeptidyl peptidase IV (DPP IV); Endothelial cell; Lysosomes; Protein A-gold technique; Rat hepatocyte;

D O I：

10.1247/csf.15.117

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated ultrastructural localization of dipeptidyl peptidase IV (DPP IV) [EC3.4.14 5] in rat liver cells quantitatively by post embedding protein A-gold technique. In the hepatocyte, DPP IV was mainly localized on the bile canalicular surface and the lysosomal membranes, but were scarcely detectable on the sinusoid-lateral surface. A small number of DPP IV was also detected in the trans region of the Golgi apparatus, suggesting that this part may play important roles in intracellular transport or recycling of this enzyme. In the endothelial cell, DPP IV existed on the whole surface of the plasma membrane and the lysosomes. In the Kupffer cell DPP IV was mainly localized in lysosomes and a few were detected on the plasma membrane. © 1990, Japan Society for Cell Biology. All rights reserved.

引用

页码：117 / 125

页数：9

共 25 条

[1] BIOGENESIS OF THE RAT HEPATOCYTE PLASMA-MEMBRANE INVIVO - COMPARISON OF THE PATHWAYS TAKEN BY APICAL AND BASOLATERAL PROTEINS USING SUBCELLULAR FRACTIONATION [J].