ISOLATION OF VINDOLINE FROM CATHARANTHUS-ROSEUS BY SUPERCRITICAL FLUID EXTRACTION

被引：27

作者：

SONG, KM

PARK, SW

HONG, WH

LEE, H

KWAK, SS

LIU, JR

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,BIOPROC ENGN RES CTR,DAEJON,SOUTH KOREA

[2] GENET ENGN RES INST,PLANT CELL BIOL LAB,DAEJON,SOUTH KOREA

来源：

BIOTECHNOLOGY PROGRESS | 1992年 / 8卷 / 06期

关键词：

D O I：

10.1021/bp00018a018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vindoline was extracted from the leaves of Catharanthus roseus over the ranges of 35-70-degrees-C and 100-300 bar using supercritical carbon dioxide with and without the addition of 3 wt % ethanol as a cosolvent. The vindoline contents in the extracts were determined by HPLC and identified by LC/MS. The remarkable highest vindoline concentration, 58 wt %, was obtained at the lowest temperature, 35-degrees-C, and the highest pressure, 300 bar, of this study. The use of a cosolvent only slightly improved the extraction yields or selectivities at some experimental conditions.

引用

页码：583 / 586

页数：4