KINETICS OF CELL INFILTRATION AND CYTOKINE MESSENGER-RNA EXPRESSION AFTER INTRADERMAL CHALLENGE WITH ALLERGEN AND TUBERCULIN IN THE SAME ATOPIC INDIVIDUALS

被引:89
作者
TSICOPOULOS, A
HAMID, Q
HACZKU, A
JACOBSON, MR
DURHAM, SR
NORTH, J
BARKANS, J
CORRIGAN, CJ
MENG, Q
MOQBEL, R
KAY, AB
机构
[1] NATL HEART & LUNG INST,DEPT ALLERGY & CLIN IMMUNOL,LONDON SW3 6LY,ENGLAND
[2] ROYAL BROMPTON NATL HEART & LUNG INST,DEPT LUNG PATHOL,LONDON,ENGLAND
基金
英国惠康基金;
关键词
IMMUNOCYTOCHEMISTRY; KINETICS; LATE-PHASE RESPONSE; LYMPHOCYTE; EOSINOPHIL; CYTOKINE;
D O I
10.1016/0091-6749(94)90185-6
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Previous studies, in which one time point was used, have shown that cells infiltrating skin biopsy specimens taken during allergen-induced late-phase responses (LPR) had a T-H2-like (interleukin-4 [IL]-4 and IL-5 mRNA(+)) cytokine profile, whereas in delayed-type hypersensitivity (DTH) there was a predominant T-H1-type pattern. Objective: The study was designed to examine the kinetics of accumulation of inflammatory cells and cells expressing mRNA for T-H2- or T-H1-type cytokines in LPR and DTH elicited simultaneously in the same subjects. Methods: Immunocytochemistry (alkaline phosphatase anti-alkaline phosphatase technique) and in situ hybridization were used to analyze skin biopsy specimens taken during allergen-induced LPR. Results: In LPR elevated numbers of CD3(+) and CD4(+) cells, eosinophils, neutrophils, and IL-4 and IL-5 mRNA(+) cells were detected as early as 1 hour after allergen challenge, with a peak at 6 hours, which was maintained for rtp to 96 hours. A small bur significant delayed increase in macrophages, CD8(+) and CD25(+) cells, and IL-2 and interferon-gamma mRNA(+) cells was also observed, but only at the 48-hour and 96-hour time points. In contrast, in DTH the numbers of CD3(+), CD4(+), and mRNA(+) cells for IL-2 and interferon-gamma were not elevated until 24 hours after challenge and peaked at 48 hours after injection. At 48 hours there was an additional small but significant increase in IL-4 and IL-5 mRNA(+) cells. For both LPR and DTH the kinetics of the increases in inflammatory cells and cytokine mRNA-expressing cells paralleled the clinical response. Conclusions: In LPR accumulation of T cells and granulocytes, together with cells expressing mRNA encoding for T-H2-type cytokines, is relatively rapid (i.e., within 1 to 6 hours), whereas in DTH the T cell/macrophage infiltration and appearance of cells expressing T-H1-type cytokines are nor apparent until 24 to 48 hours. In LPR there is a T-H1-type (or possibly T-H0) component at 48 to 96 hours, and in DTH there is an additional T-H2/T-H0 response.
引用
收藏
页码:764 / 772
页数:9
相关论文
共 25 条
[1]   INTERLEUKIN-4 IS LOCALIZED TO AND RELEASED BY HUMAN MAST-CELLS [J].
BRADDING, P ;
FEATHER, IH ;
HOWARTH, PH ;
MUELLER, R ;
ROBERTS, JA ;
BRITTEN, K ;
BEWS, JPA ;
HUNT, TC ;
OKAYAMA, Y ;
HEUSSER, CH ;
BULLOCK, GR ;
CHURCH, MK ;
HOLGATE, ST .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (05) :1381-1386
[2]  
BROD SA, 1991, J IMMUNOL, V147, P810
[3]   EOSINOPHILS EXPRESS INTERLEUKIN-5 AND GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR MESSENGER-RNA AT SITES OF ALLERGIC INFLAMMATION IN ASTHMATICS [J].
BROIDE, DH ;
PAINE, MM ;
FIRESTEIN, GS .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (04) :1414-1424
[4]   PURIFIED PROTEIN DERIVATIVE OF MYCOBACTERIUM-TUBERCULOSIS AND EXCRETORY-SECRETORY ANTIGEN(S) OF TOXOCARA-CANIS EXPAND INVITRO HUMAN T-CELLS WITH STABLE AND OPPOSITE (TYPE-1 T-HELPER OR TYPE-2 T-HELPER) PROFILE OF CYTOKINE PRODUCTION [J].
DELPRETE, GF ;
DECARLI, M ;
MASTROMAURO, C ;
BIAGIOTTI, R ;
MACCHIA, D ;
FALAGIANI, P ;
RICCI, M ;
ROMAGNANI, S .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (01) :346-350
[5]   INTERLEUKIN-5 MESSENGER-RNA EXPRESSION BY EOSINOPHILS IN THE INTESTINAL-MUCOSA OF PATIENTS WITH CELIAC-DISEASE [J].
DESREUMAUX, P ;
JANIN, A ;
COLOMBEL, JF ;
PRIN, L ;
PLUMAS, J ;
EMILIE, D ;
TORPIER, G ;
CAPRON, A ;
CAPRON, M .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (01) :293-296
[6]  
FREW AJ, 1988, J IMMUNOL, V141, P4158
[7]  
GAGA M, 1991, J IMMUNOL, V147, P816
[8]  
GAJEWSKI TF, 1988, J IMMUNOL, V140, P4245
[9]  
HAENEN JBA, 1991, J EXP MED, V174, P583
[10]   LOCALIZATION OF ATRIAL-NATRIURETIC-PEPTIDE MESSENGER-RNA AND IMMUNOREACTIVITY IN THE RAT-HEART AND HUMAN ATRIAL APPENDAGE [J].
HAMID, Q ;
WHARTON, J ;
TERENGHI, G ;
HASSALL, CJS ;
AIMI, J ;
TAYLOR, KM ;
NAKAZATO, H ;
DIXON, JE ;
BURNSTOCK, G ;
POLAK, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6760-6764