PURIFICATION AND CHARACTERIZATION OF A MALTASE FROM THE EXTREMELY THERMOPHILIC CRENARCHAEOTE SULFOLOBUS-SOLFATARICUS

被引：90

作者：

ROLFSMEIER, M ^{[1
]}

BLUM, P ^{[1
]}

机构：

[1] UNIV NEBRASKA,SCH BIOL SCI,LINCOLN,NE 68588

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 02期

关键词：

D O I：

10.1128/jb.177.2.482-485.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A soluble maltase (alpha-glucosidase) with an apparent subunit mass of 80 kDa was purified to homogeneity from Sulfolobus solfataricus. The enzyme liberates glucose from maltose and malto-oligomers. Maximal activity was observed at 105 degrees C, with half-lives of 11 h (85 degrees C), 3.0 h (95 degrees C), and 2.75 h (100 degrees C). The enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-Propanol treatment at 85 degrees C increased both K-m and V-max for maltose hydrolysis.

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页码：482 / 485

页数：4

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