INFLUENCE OF THE 2ND AND 3RD CODON ON THE EXPRESSION OF RECOMBINANT HIRUDIN IN ESCHERICHIA-COLI

被引：3

作者：

DEGRYSE, E

机构：

[1] Transgene S.A., 67000 Strasbourg

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

Codon usage; E; coli; Expression; Recombinant hirudin;

D O I：

10.1016/0014-5793(90)81164-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product accumulation follows the introduction for ile of the ATA codon which is of very low usage, and for thr of the ACC codon, which results in homology of the mRNA with the 3'-end of 16S rRNA. The results are discussed according to current concepts of protein expression in E. coli. © 1990.

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页码：244 / 246

页数：3

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