ISOLATION AND CHARACTERIZATION OF SOMATOSTATIN FROM ANGLERFISH PANCREATIC-ISLET

被引：90

作者：

NOE, BD

SPIESS, J

RIVIER, JE

VALE, W

机构：

[1] MARINE BIOL LAB,WOODS HOLE,MA 02543

[2] SALK INST BIOL STUDIES,PEPTIDE BIOL LAB,SAN DIEGO,CA 92112

来源：

ENDOCRINOLOGY | 1979年 / 105卷 / 06期

关键词：

D O I：

10.1210/endo-105-6-1410

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Somatostatin was purified from anglerfish pancreatic islets using acetic acid extraction, gel filtration (Bio-Gel P-10), ion exchange chromatography (CM Bio-Gel A), and reversed phase high pressure liquid chromatography. The resulting peptide was characterized by RIA, bioassay, and determination of amino acid composition. Anglerfish islet somatostatin was found to possess an amino acid composition and immunological and biological activities equivalent to synthetic somatostatin. Sequence analyses revealed that the primary structure was HAla- Gly-cyclo-[Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys]-OH. These results demonstrate that anglerfish islet omatostatin has the same primary structure as somatostatin from all other sources characterized to date. © 1979 by The Endocrine Society.

引用

收藏

页码：1410 / 1415

页数：6

相关论文

共 23 条

[1] RADIOIMMUNOASSAY OF SOMATOSTATIN [J].

ARIMURA, A ;

LUNDQVIST, G ;

ROTHMAN, J ;

CHANG, R ;

FERNANDEZDURANGO, R ;

ELDE, R ;

COY, DH ;

MEYERS, C ;

SCHALLY, AV .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1978, 27 (09) :1139-1144

[2] HYPOTHALAMIC POLYPEPTIDE THAT INHIBITS SECRETION OF IMMUNOREACTIVE PITUITARY GROWTH-HORMONE [J].

BRAZEAU, P ;

VALE, W ;

BURGUS, R ;

LING, N ;

BUTCHER, M ;

RIVIER, J ;

GUILLEMIN, R .

SCIENCE, 1973, 179 (4068) :77-79

[3] PRIMARY STRUCTURE OF SOMATOSTATIN - HYPOTHALAMIC PEPTIDE THAT INHIBITS SECRETION OF PITUITARY GROWTH-HORMONE [J].

BURGUS, R ;

LING, N ;

BUTCHER, M ;

GUILLEMI.R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (03) :684-688

[4]

Burgus R, 1976, PEPTIDES 1976, P85

[5]

FALKMER S, 1976, DIABETIC PANCREAS

[6] LOCALIZATION OF SOMATOSTATIN-LIKE AND GASTRIN-LIKE IMMUNOREACTIVITY IN GASTROINTESTINAL-TRACT OF CIONA-INTESTINALIS L [J].

FRITSCH, HAR ;

VANNOORDEN, S ;

PEARSE, AGE .

CELL AND TISSUE RESEARCH, 1978, 186 (01) :181-185

[7] DIRECT MICROSEQUENCE ANALYSIS OF POLYPEPTIDES USING AN IMPROVED SEQUENATOR, A NON-PROTEIN CARRIER (POLYBRENE), AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

HUNKAPILLER, MW ;

HOOD, LE .

BIOCHEMISTRY, 1978, 17 (11) :2124-2133

[8] IMMUNOHISTOCHEMICAL LOCALIZATION OF SOMATOSTATIN, INSULIN AND GLUCAGON IN PRINCIPAL ISLETS OF ANGLERFISH (LOPHIUS-AMERICANUS) AND CHANNEL CATFISH (ICTALURUS-PUNCTATA) [J].

JOHNSON, DE ;

TORRENCE, JL ;

ELDE, RP ;

BAUER, GE ;

NOE, BD ;

FLETCHER, DJ .

AMERICAN JOURNAL OF ANATOMY, 1976, 147 (01) :119-124

[9] MICROPOLYAMIDE THIN-LAYER CHROMATOGRAPHY OF PHENYLTHIOHYDANTOIN AMINO-ACIDS (PTH) AT SUBNANOMOLAR LEVEL - RAPID MICROTECHNIQUE FOR SIMULTANEOUS MULTISAMPLE IDENTIFICATION AFTER AUTOMATED EDMAN DEGRADATIONS [J].

KULBE, KD .

ANALYTICAL BIOCHEMISTRY, 1974, 59 (02) :564-573

[10] SOMATOSTATIN - BOTH HORMONE AND NEUROTRANSMITTER [J].

LUFT, R ;

EFENDIC, S ;

HOKFELT, T .

DIABETOLOGIA, 1978, 14 (01) :1-13