LIQUID-CHROMATOGRAPHIC DETERMINATION OF N-HYDROXY-3,4-METHYLENEDIOXYAMPHETAMINE AND METABOLITE IN PLASMA

The analysis of N-hydroxy-3,4-methylenedioxyamphetaiTTine (NOHMDA) from plasma samples using reversed-phase liquid chromatography is described. The separation of NOHMDA, its metabolite 3,4-methylenedioxyamphetamine (MDA) and the internal standard N-methyl-MDA (MDMA) were well resolved in about twelve minutes using a CB stationary phase and an acidic aqueous acetonitrile mobile phase. The isolation procedure involved liquid/liquid extraction of 50 uL of plasma using chloroform followed by HPLC analysis of the resulting residue. The recovery was greater than 90% for NOHMDA and slightly higher for MDA with good reproductibility. © 1990, Taylor & Francis Group, LLC. All rights reserved.