FLOW-INJECTION ELECTROCHEMICAL ENZYME-IMMUNOASSAY FOR THEOPHYLLINE USING A PROTEIN-A IMMUNOREACTOR AND P-AMINOPHENYL PHOSPHATE-P-AMINOPHENOL AS THE DETECTION SYSTEM

被引：43

作者：

PALMER, DA

EDMONDS, TE

SEARE, NJ

机构：

来源：

ANALYST | 1992年 / 117卷 / 11期

关键词：

ELECTROCHEMICAL DETECTION; ENZYME IMMUNOASSAY; FLOW INJECTION; THEOPHYLLINE; PROTEIN-A;

D O I：

10.1039/an9921701679

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A competitive electrochemical enzyme immunoassay has been developed for the antiasthmatic drug theophylline, utilizing a controlled-pore glass-protein A immunoreactor and flow injection techniques. p-Aminophenyl phosphate, a substrate for alkaline phosphatase, has been used in this assay, and its hydrolysis product p-aminophenol was determined at +0.2 V versus the saturated calomel electrode. For each sample the antibody-protein A reaction takes place at near-neutral pH, and the complexes are eluted at acid pH. Serum theophylline has been determined by this method, and good relative standard deviations and percentage recoveries have been achieved.

引用

页码：1679 / 1682

页数：4

共 42 条

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