DEOXYRIBOCYTIDINE IS SALVAGED NOT ONLY INTO DNA BUT ALSO INTO PHOSPHOLIPID PRECURSORS .4. EXOGENOUS DEOXYRIBOCYTIDINE CAN BE USED WITH THE SAME EFFICACY AS (RIBO)CYTIDINE FOR LIPID ACTIVATION

被引：13

作者：

SASVARISZEKELY, M

SPASOKUKOTSKAJA, T

STAUB, M

机构：

[1] 1st Institute of Biochemistry, Semmehweis Medical University, H-1444 Budapest 8

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Utilisation of exogenous (ribo)cytidine (3H-CR) and deoxyribocytidine (3H-CdR) for DNA/RNA synthesis and for activation of phospholipid intermediates was compared in human tonsillar lymphocytes. Incorporation of 3H-CdR into dCDP-choline and into dCDP-DAG was similar or even higher than labelling of CDP-choline and CDP-DAG from 3H-CR. No interconversion was found between CDP-DAG and dCDP-DAG, as shown by TLC separation of the ribo- and deoxyribocytidine derivatives. Moreover, a strict separation was found between the salvage pathways of deoxyribocytidine and (ribo)cytidine, as 4000-fold excess of non labelled (ribo)cytidine did not show any specific effect on 3H-dCDP-DAG labelling from exogenous 3HCdR. © 1993 Academic Press, Inc.

引用

页码：966 / 972

页数：7

共 20 条

[1] DEOXYCYTIDINE KINASE IS CONSTITUTIVELY EXPRESSED IN HUMAN-LYMPHOCYTES - CONSEQUENCES FOR COMPARTMENTATION EFFECTS, UNSCHEDULED DNA-SYNTHESIS, AND VIRAL REPLICATION IN RESTING CELLS [J].