Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

被引:109
作者
Pedersen, Carsten [1 ]
Linde-Laursen, Ib [1 ]
机构
[1] Riso Natl Lab, Environm Sci & Technol Dept, Plant Biol Sect, DK-4000 Roskilde, Denmark
关键词
C-banding; chromosome identification; Hordeum vulgare; in situ hybridization; microsatellite;
D O I
10.1007/BF01539456
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (71), 2 (21), 4 (41), and 5 (11) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.
引用
收藏
页码:65 / 71
页数:7
相关论文
共 35 条
[1]   BEHAVIOR OF PARENTAL GENOMES IN THE HYBRID HORDEUM-VULGARE X H-BULBOSUM [J].
ANAMTHAWATJONSSON, K ;
SCHWARZACHER, T ;
HESLOPHARRISON, JS .
JOURNAL OF HEREDITY, 1993, 84 (01) :78-82
[2]   CHROMOSOMAL ASSIGNMENT OF HUMAN YAC CLONES BY FLUORESCENCE INSITU HYBRIDIZATION - USE OF SINGLE-YEAST-COLONY PCR AND MULTIPLE LABELING [J].
BALDINI, A ;
ROSS, M ;
NIZETIC, D ;
VATCHEVA, R ;
LINDSAY, EA ;
LEHRACH, H ;
SINISCALCO, M .
GENOMICS, 1992, 14 (01) :181-184
[3]  
Baldini A, 1992, GENOMICS, V9, P770
[4]   PRELIMINARY KARYOTYPE AND CHROMOSOMAL LOCALIZATION OF RIBOSOMAL DNA SITES IN WHITE SPRUCE USING FLUORESCENCE INSITU HYBRIDIZATION [J].
BROWN, GR ;
AMARASINGHE, V ;
KISS, G ;
CARLSON, JE .
GENOME, 1993, 36 (02) :310-316
[5]   DETECTION OF SINGLE-COPY GENES BY NONISOTOPIC INSITU HYBRIDIZATION ON HUMAN-CHROMOSOMES [J].
CHERIF, D ;
BERNARD, O ;
BERGER, R .
HUMAN GENETICS, 1989, 81 (04) :358-362
[6]   SIMULTANEOUS LOCALIZATION OF COSMIDS AND CHROMOSOME R-BANDING BY FLUORESCENCE MICROSCOPY - APPLICATION TO REGIONAL MAPPING OF HUMAN CHROMOSOME-11 [J].
CHERIF, D ;
JULIER, C ;
DELATTRE, O ;
DERRE, J ;
LATHROP, GM ;
BERGER, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6639-6643
[7]   IDENTICAL POLYPYRIMIDINE-POLYPURINE SATELLITE DNAS IN WHEAT AND BARLEY [J].
DENNIS, ES ;
GERLACH, WL ;
PEACOCK, WJ .
HEREDITY, 1980, 44 (JUN) :349-+
[8]  
DOLEZEL J, 1992, PLANTA, V188, P93, DOI 10.1007/BF00198944
[9]   MAPPING SMALL DNA-SEQUENCES BY FLUORESCENCE INSITU HYBRIDIZATION DIRECTLY ON BANDED METAPHASE CHROMOSOMES [J].
FAN, YS ;
DAVIS, LM ;
SHOWS, TB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6223-6227
[10]  
Flavell R. B., 1982, Genome evolution, P301