ABSENCE OF IMMUNOREACTIVE CYTOKINES IN SUPERNATANTS OF INDIVIDUAL PREIMPLANTATION HUMAN EMBRYOS

被引：21

作者：

SEIFER, DB ^{[1
]}

ROMERO, R ^{[1
]}

BERLINSKY, D ^{[1
]}

HANING, RV ^{[1
]}

机构：

[1] WAYNE STATE UNIV,HUTZEL HOSP,SCH MED,DEPT OBSTET & GYNECOL,DETROIT,MI 48202

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1993年 / 30卷 / 2-3期

关键词：

CYTOKINES; HUMAN PREIMPLANTATION EMBRYOS; ELISA;

D O I：

10.1111/j.1600-0897.1993.tb00610.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

引用

页码：105 / 107

页数：3