DISTINCT INTRACELLULAR FATES OF MEMBRANE AND SECRETORY IMMUNOGLOBULIN HEAVY-CHAINS IN A PRE-B CELL-LINE

被引:14
作者
BACHHAWAT, AK [1 ]
PILLAI, S [1 ]
机构
[1] HARVARD UNIV,SCH MED,BOSTON,MA 02129
关键词
D O I
10.1083/jcb.115.3.619
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The intracellular fates of membrane and secretory immunoglobulin heavy chains were examined in a pre-B cell line that has switched to the gamma-isotype. The membrane form of the heavy chain (gamma-m) was rapidly degraded while the secretory form (gamma-s) was retained intracellularly in association with BiP. The degradation of gamma-m could not be inhibited by ammonium chloride, chloroquine, or monensin suggesting that it occurred in a nonlysosomal compartment. The inability to detect any Endo H-resistant form of gamma-m before its degradation suggested that degradation occurs before entry into the Golgi compartment. Degradation of gamma-m could be inhibited by incubation at 24-degrees-C. In a derivative of this cell line expressing a transfected kappa gene, gamma-s formed disulfide linked tetramers with kappa and was secreted, while gamma-m, although associated with kappa, continued to be rapidly degraded. These observations suggest that membrane and secretory heavy chain proteins are retained by distinct intracellular mechanisms. Although masking of the CH1 domain abrogates gamma-s retention, this domain does not influence the intracellular fate of gamma-m.
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页码:619 / 624
页数:6
相关论文
共 51 条
[1]   INTRACELLULAR DEGRADATION OF UNASSEMBLED ASIALOGLYCOPROTEIN RECEPTOR SUBUNITS - A PRE-GOLGI, NONLYSOSOMAL ENDOPROTEOLYTIC CLEAVAGE [J].
AMARA, JF ;
LEDERKREMER, G ;
LODISH, HF .
JOURNAL OF CELL BIOLOGY, 1989, 109 (06) :3315-3324
[2]   POSTTRANSLATIONAL ASSOCIATION OF IMMUNOGLOBULIN HEAVY-CHAIN BINDING-PROTEIN WITH NASCENT HEAVY-CHAINS IN NONSECRETING AND SECRETING HYBRIDOMAS [J].
BOLE, DG ;
HENDERSHOT, LM ;
KEARNEY, JF .
JOURNAL OF CELL BIOLOGY, 1986, 102 (05) :1558-1566
[3]   PRE-GOLGI DEGRADATION OF NEWLY SYNTHESIZED T-CELL ANTIGEN RECEPTOR CHAINS - INTRINSIC SENSITIVITY AND THE ROLE OF SUBUNIT ASSEMBLY [J].
BONIFACINO, JS ;
SUZUKI, CK ;
LIPPINCOTTSCHWARTZ, J ;
WEISSMAN, AM ;
KLAUSNER, RD .
JOURNAL OF CELL BIOLOGY, 1989, 109 (01) :73-83
[4]   COLOCALIZED TRANSMEMBRANE DETERMINANTS FOR ER DEGRADATION AND SUBUNIT ASSEMBLY EXPLAIN THE INTRACELLULAR FATE OF TCR CHAINS [J].
BONIFACINO, JS ;
COSSON, P ;
KLAUSNER, RD .
CELL, 1990, 63 (03) :503-513
[5]   IMMUNOGLOBULIN HEAVY-CHAIN CLASS SWITCHING IN A PRE-B CELL-LINE IS ACCOMPANIED BY DNA REARRANGEMENT [J].
BURROWS, PD ;
BECKENGESER, GB ;
WABL, MR .
NATURE, 1983, 306 (5940) :243-246
[6]   SELECTIVE DEGRADATION OF T-CELL ANTIGEN RECEPTOR CHAINS RETAINED IN A PRE-GOLGI COMPARTMENT [J].
CHEN, C ;
BONIFACINO, JS ;
YUAN, LC ;
KLAUSNER, RD .
JOURNAL OF CELL BIOLOGY, 1988, 107 (06) :2149-2161
[7]   DIFFERENCES IN GLYCOPROTEIN COMPLEXES ASSOCIATED WITH IGM AND IGD ON NORMAL MURINE B-CELLS POTENTIALLY ENABLE TRANSDUCTION OF DIFFERENT SIGNALS [J].
CHEN, JZ ;
STALL, AM ;
HERZENBERG, LA ;
HERZENBERG, LA .
EMBO JOURNAL, 1990, 9 (07) :2117-2124
[8]   THE OMEGA/DELTA-5-SURROGATE IMMUNOGLOBULIN LIGHT CHAIN IS EXPRESSED ON THE SURFACE OF TRANSITIONAL LYMPHOCYTES-B IN MURINE BONE-MARROW [J].
CHERAYIL, BJ ;
PILLAI, S .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (01) :111-116
[9]   BREFELDIN-A REDISTRIBUTES RESIDENT AND ITINERANT GOLGI PROTEINS TO THE ENDOPLASMIC-RETICULUM [J].
DOMS, RW ;
RUSS, G ;
YEWDELL, JW .
JOURNAL OF CELL BIOLOGY, 1989, 109 (01) :61-72
[10]  
FINDLEY HW, 1982, BLOOD, V60, P1305