The principal volatile products of the formicine ant Lasius alienus were identified, their glandular sources located in part, and their functions analysed. The more abundant substances in the C9-C10 range were shown to be alarm substances, with behavioral threshold concentrations estimated to vary in order of magnitude from 107 to 1011 molecules/cm3. Undecane, the principal component, was shown to be more effective as an alarm substance for L. alienus, whose estimated response threshold is 107 to 1010 molecules/cm3, than for the relazed species Acanthomyops claviger, whose estimated threshold is 1010 to 1012 molecules/cm3. L. alienus appears to depend less in its defence strategy on orientation toward points of disturbances and defensive chemical sprays, and more on 'early warning' and evacuation, than does A. claviger. © 1969.