ASSAY OF CATECHOL O-METHYLTRANSFERASE BY DETERMINATION OF THE META-O-METHYLATED AND PARA-O-METHYLATED PRODUCTS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：23

作者：

PENNINGS, EJM

VANKEMPEN, GMJ

机构：

[1] Department of Biochemistry, Psychiatric Institute Endegeest

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90166-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new assay of catechol O-methyltransferase (EC 2.1.1.6) is described by determination of the m- and p-O-methylated products of 3,4-dihydroxybenzoic acid. The method involves DEAE-Sephadex A-25 chromatography and reversed-phase high-performance liquid chromatography on a LiChrosorb 5 RP 18 column. The liquid chromatographic solvent system consisted of 0.05 m acetic acid in methanol:water (1:4, v v), pH 3,2. meta/para ratios of O-methylation are easily obtained by this method. Dimethylation has not been observed with a partially purified enzyme preparation from rat liver. © 1979.

引用

页码：452 / 454

页数：3

共 11 条

[1]

AXELROD J, 1958, J BIOL CHEM, V233, P702

[2]

AXELROD J, 1958, J BIOL CHEM, V233, P697

[3]

BROWN PR, 1973, HIGH PRESSURE LIQUID, P124

[4]

CREVELING CR, 1970, MOL PHARMACOL, V6, P691