FLUOROMETRIC-DETERMINATION OF PYRUVIC-ACID AND ALPHA-KETOGLUTARIC ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14

作者：

NAKAMURA, H

TAMURA, Z

机构：

[1] Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 11期

关键词：

D O I：

10.1021/ac50047a021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A HPLC [high performance liquid chromatography] method was developed for the fluorometric determination of pyruvic acid (PA) and .alpha.-ketoglutaric acid (.alpha.-KG). They were separated by anion-exchange chromatography, reacted with N1-methylnicotinamide chloride (NMN) in the presence of alkali, and heated after the acidification with formic acid to produce fluorophores. By using the optimized conditions for chromatography and the post-column derivatzation, 15 pmol of PA and 75 pmol of .alpha.-KG could be determined. The relative standard deviations of the method were 3.82% and 3.32% for the analyses of 500 pmol each of PA and .alpha.-KG, respectively.

引用

页码：1679 / 1683

页数：5

共 17 条

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