LOCALIZATION OF BASIC FGF-LIKE IMMUNOREACTIVITY IN THE HYPOTHALAMOHYPOPHYSEAL NEUROENDOCRINE AXIS

被引:37
作者
IWATA, H
MATSUYAMA, A
OKUMURA, N
YOSHIDA, S
LEE, Y
IMAIZUMI, K
SHIOSAKA, S
机构
[1] OSAKA UNIV,SCH MED,BIOMED RES CTR,DEPT NEUROANAT,4-3-57 NAKANOSHIMA,KITA KU,OSAKA 530,JAPAN
[2] TANABE SEIYAKU CO LTD,OSAKA 532,JAPAN
[3] EHIME UNIV,SCH MED,DEPT BIOCHEM,MATSUYAMA,EHIME 790,JAPAN
关键词
BASIC FIBROBLAST GROWTH FACTOR; HYPOTHALAMUS; HYPOPHYSIS; IMMUNOHISTOCHEMISTRY; WESTERN-BLOTTING;
D O I
10.1016/0006-8993(91)91336-Y
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We examined the localization of basic fibroblast growth factor (basic FGF) in the adult rat brain by immunohistochemical and Western blotting analysis using a specific antibody against a synthetic basic FGF fragment (N-terminal 12 residues). The antibody did not cross-react with acidic FGF. Basic FGF-like immunoreactivity was located exclusively in the neuronal elements and had very heterogeneous distribution. Immunoreactive cell bodies were observed in the paraventricular, supraoptic and circular nuclei of the hypothalamus. Numerous immunoreactive neuronal processes originating from these basic FGF-positive cells extended lateroventrally and then caudally to the internal layer of the median eminence. In addition, the neurohypophysis contained a significant number of basic FGF-like immunoreactive fibers. Western-blotting analysis revealed that the hypothalamus and the hypophysis contained a main band of basic FGF immunoreactive with an apparent molecular weight of 17 kDa. These results show that the hypothalamo-hypophyseal neuroendocrine pathway contains basic FGF.
引用
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页码:329 / 332
页数:4
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