A SAFER PROCEDURE FOR ROUTINE STAINING OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI

被引：266

作者：

GRACE, C

STRIBLEY, DP

机构：

[1] Soil Science Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire

来源：

MYCOLOGICAL RESEARCH | 1991年 / 95卷

关键词：

D O I：

10.1016/S0953-7562(09)80005-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Trypan blue is a popular stain for vesicular-arbuscular mycorrhizal fungi but it is a possible human carcinogen. It is often used in solution in lactophenol, another hazardous chemical. Current safety regulations (Control of Substances Hazardous to Health Regulations 1988: COSHH) require that where substances hazardous to health are currently used, safer substances should be substituted as far as possible. We describe a method for routine staining of large numbers of samples of mycorrhizas which uses the less hazardous combination of aniline blue or methyl blue and glycerol.

引用

页码：1160 / 1162

页数：3

共 17 条

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