APPLICATIONS OF STABLE V79-DERIVED CELL-LINES EXPRESSING RAT CYTOCHROMES P4501A1, 1A2, AND 2B1

被引：19

作者：

DOEHMER, J ^{[1
]}

WOLFEL, C ^{[1
]}

DOGRA, S ^{[1
]}

DOEHMER, C ^{[1
]}

SEIDEL, A ^{[1
]}

PLATT, KL ^{[1
]}

OESCH, F ^{[1
]}

GLATT, HR ^{[1
]}

机构：

[1] UNIV MAINZ,W-6500 MAINZ,GERMANY

来源：

XENOBIOTICA | 1992年 / 22卷 / 9-10期

关键词：

D O I：

10.3109/00498259209051863

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Chinese hamster V79-derived cell lines, stably expressing cytochromes P4501A1, 1A2, and 2B1 activities, were constructed by genetic engineering in continuation of our work to establish a battery of V79 derived cell lines designed to study the metabolism of xenobiotics. 2. Cell lines XEM1 and XEM2, expressing cytochrome P4501A1, were capable of the O-dealkylation of 7-ethoxycoumarin and the hydroxylation of benzo[a]pyrene. 3. Cell lines XEMd.MZ and XEMd.NH, expressing P4501A2, were shown to hydroxylate 17beta-estradiol and 2-aminofluorene. 4. Cell line SD1, expressing cytochrome P4502B1, was able to hydroxylate testosterone stereo- and regio-specifically at the 16alpha and 16beta positions. 5. Cell lines were validated in mutagenicity, cytotoxicity, and metabolism studies employing benzo[a]pyrene, trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, cyclophosphamide, ifosfamide, and picene. 6. Construction of metabolically-competent V79-derived cell lines be recombinant DNA technology will be a fundamental improvement for the evaluation of the cytotoxic, genotoxic and pharmacological properties of a chemical,

引用

页码：1093 / 1099

页数：7

共 16 条

[1] AOYAMA T, 1990, DRUG METAB DISPOS, V18, P378
[2] AOYAMA T, 1989, MOL CARCINOGEN, V2, P1925
[3] BATTULA N, 1989, J BIOL CHEM, V264, P2991
[4] CLARKE L, 1989, CANCER RES, V49, P2344
[5] DEVELOPMENT OF A HUMAN CELL-LINE BY SELECTION AND DRUG-METABOLIZING GENE TRANSFECTION WITH INCREASED CAPACITY TO ACTIVATE PROMUTAGENS
DAVIES, RL
CRESPI, CL
RUDO, K
TURNER, TR
LANGENBACH, R
[J]. CARCINOGENESIS, 1989, 10 (05) : 885 - 891
[6] INTRODUCTION OF RAT GROWTH-HORMONE GENE INTO MOUSE FIBROBLASTS VIA A RETROVIRAL DNA VECTOR - EXPRESSION AND REGULATION
DOEHMER, J
BARINAGA, M
VALE, W
ROSENFELD, MG
VERMA, IM
EVANS, RM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (07): : 2268 - 2272
[7] STABLE EXPRESSION OF RAT CYTOCHROME P-450IIB1 CDNA IN CHINESE-HAMSTER CELLS (V79) AND METABOLIC-ACTIVATION OF AFLATOXIN-B1
DOEHMER, J
DOGRA, S
FRIEDBERG, T
MONIER, S
ADESNIK, M
GLATT, H
OESCH, F
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (16) : 5769 - 5773
[8] GENETICALLY ENGINEERED V79 CHINESE HAMSTER-CELLS METABOLICALLY ACTIVATE THE CYTOSTATIC DRUGS CYCLOPHOSPHAMIDE AND IFOSFAMIDE
DOEHMER, J
SEIDEL, A
OESCH, F
GLATT, HR
[J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 1990, 88 : 63 - 65
[9] DOGRA S, 1990, MOL PHARMACOL, V37, P608
[10] METABOLIC-ACTIVATION TO A MUTAGEN OF 3-HYDROXY-TRANS-7,8-DIHYDROXY-7,8-DIHYDROBENZO[A]PYRENE, A SECONDARY METABOLITE OF BENZO[A]PYRENE
GLATT, H
SEIDEL, A
RIBEIRO, O
KIRKBY, C
HIROM, P
OESCH, F
[J]. CARCINOGENESIS, 1987, 8 (11) : 1621 - 1627

← 1 2 →