A LINKAGE MAP FOR SUGI (CRYPTOMERIA-JAPONICA) BASED ON RFLP, RAPD, AND ISOZYME LOCI

被引：46

作者：

MUKAI, Y

SUYAMA, Y

TSUMURA, Y

KAWAHARA, T

YOSHIMARU, H

KONDO, T

TOMARU, N

KURAMOTO, N

MURAI, M

机构：

[1] FOREST TREE BREEDING INST,MITO,IBARAKI 310,JAPAN

[2] UNIV TSUKUBA,INST AGR & FORESTRY,TSUKUBA,IBARAKI 305,JAPAN

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 06期

关键词：

RFLP; RAPD; LINKAGE MAP; SUGI (CRYPTOMERIA JAPONICA); 3-GENERATION; PEDIGREE;

D O I：

10.1007/BF00222019

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

引用

页码：835 / 840

页数：6

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