BIODEGRADATION OF PESTICIDES IN NONIONIC WATER-IN-OIL MICROEMULSIONS OF TWEEN-85 - RELATIONSHIP BETWEEN MICELLE STRUCTURE AND ACTIVITY

被引:26
作者
KOMIVES, CF [1 ]
LILLEY, E [1 ]
RUSSELL, AJ [1 ]
机构
[1] UNIV PITTSBURGH,CTR BIOTECHNOL & BIOENGN,PITTSBURGH,PA 15261
关键词
ENZYMES; PHOSPHOTRIESTERASE; REVERSED MICELLES; MICROEMULSIONS; NONIONIC SURFACTANTS; ORGANOPHOSPHORUS HYDROLASE;
D O I
10.1002/bit.260431008
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Water-in-oil microemulsion systems have been studied in recent years for a number of applications in protein separation and enzymology. Although it is well established that reversed micelle systems provide an excellent medium for nonaqueous biocatalytic studies, there is still much speculation as to the interaction of the enzyme with the surfactant interface. Polyoxyethylene sorbitan trioleate (Tween 85) is a nonionic surfactant which has some interesting properties for microemulsion formation and protein solubilization. In conjunction with a separate article describing the structural features of Tween 85 reversed micelles in hexane with isopropanol as a cosurfactant, this work describes the activity of an enzyme, organophosphorus hydrolase, for degrading organophosphorus pesticides in this microemulsion system. Ternary phase diagrams were constructed to outline the phase boundaries at different temperatures and isopropanol concentrations, which elucidate the role of the cosurfactant alcohol, as well as some features of micelle structure. Kinetic and stability studies with organophosphorus hydrolase show the effect of enzyme partitioning between the micelle surfactant layer and aqueous core. (C) 1994 John Wiley & Sons, Inc.
引用
收藏
页码:946 / 959
页数:14
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