THE INTRINSIC 22 KDA PROTEIN IS A CHLOROPHYLL-BINDING SUBUNIT OF PHOTOSYSTEM-II

被引：52

作者：

FUNK, C

SCHRODER, WP

GREEN, BR

RENGER, G

ANDERSSON, B

机构：

[1] UNIV STOCKHOLM,ARRHENIUS LABS NAT SCI,DEPT BIOCHEM,S-10691 STOCKHOLM,SWEDEN

[2] TECH UNIV BERLIN,MAX VOLMER INST,D-10623 BERLIN,GERMANY

[3] UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1Z4,BC,CANADA

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

CHLOROPHYLL-PROTEIN; PSBS GENE PRODUCT; 22 KDA PROTEIN; PHOTOSYSTEM II; SPINACH;

D O I：

10.1016/0014-5793(94)80513-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intrinsic 22 kDa polypeptide associated with photosystem II (psbS protein) was found to be able to bind chlorophyll. Extraction of isolated photosystem II membranes with octyl-thioglucopyranoside, followed by repetitive electrophoresis under partially denaturing conditions gave only one green band. It contained both chlorophyll a and chlorophyll b, exhibited an absorption maximum at 674 nm and a 77 K fluorescence peak at 675 Mn. The chIorophyll-protein band contained a single polypeptide of 22 kDa. Based on these results and on previous protein sequence comparisons, it is suggested that the psbS protein is a chlorophyll a/b binding polypeptide and should thus be denoted CP22.

引用

页码：261 / 266

页数：6

共 33 条

[1]

ADAMSKA I, 1993, J BIOL CHEM, V268, P5438

[2] CHLOROPHYLL-PROTEIN COMPLEXES OF SPINACH AND BARLEY THYLAKOIDS - SPECTRAL CHARACTERIZATION OF 6 COMPLEXES RESOLVED BY AN IMPROVED ELECTROPHORETIC PROCEDURE [J].