RAPID METHOD, USING OCTADECASILYL-SILICA, FOR EXTRACTION OF CERTAIN PEPTIDES FROM TISSUES

被引：88

作者：

BENNETT, HPJ ^{[1
]}

HUDSON, AM ^{[1
]}

KELLY, L ^{[1
]}

MCMARTIN, C ^{[1
]}

PURDON, GE ^{[1
]}

机构：

[1] CIBA GEIGY,CIBA LABS,HORSHAM RES CTR,DIV PHARMACEUT,WIMBLEHURST RD,HORSHAM RH12 4AB,W SUSSEX,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1978年 / 175卷 / 03期

关键词：

D O I：

10.1042/bj1751139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.

引用

页码：1139 / 1141

页数：3

共 10 条

[1] ALLEN MC, CHEM COMMUN
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[7] Burgus R, 1976, PEPTIDES 1976, P85
[8] FLUOROMETRIC ASSAY OF VASOPRESSIN AND OXYTOCIN - GENERAL APPROACH TO ASSAY OF PEPTIDES IN TISSUES
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[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (04) : 1314 - 1318
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