EFFECT OF UNCOUPLERS OF OXIDATIVE-PHOSPHORYLATION ON MICROTUBULE LOCATION AND SURFACE-STRUCTURE IN MURINE MAST-CELLS

“Uncouplers” of oxidative phosphorylation (sodium azide, DNP, and oligomycin) alter the location of microtubules within murine mast cells. Both cytoplasmic microtubuless, perpendicular to the plasma membrane within cell surface folds, and intranuclear microtubules wereobserved. In addition, one or more dense plaque‐like structures adjacent the plasma membrane in mast cells appeared following incubation in the antimetabolities. Intranuclear microtubules and cytoplasmic microtubules within the cell surface ridges disappeared in azide‐treated mast cells that were reincubated or “recovered” in PBS. However, both these structures remained in oligomycin‐ and DNP‐treated murine mast cells following reincubation. Copyright © 1979 Wiley‐Liss, Inc.