DIFFERENTIATION OF THE HEMAGGLUTININ GENES OF VARIANT INFLUENZA-VIRUSES BY RNA-RNA HYBRIDIZATION

被引：29

作者：

HAY, AJ

SKEHEL, JJ

WEBSTER, RG

机构：

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 45卷 / OCT期

关键词：

D O I：

10.1099/0022-1317-45-1-245

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genetic compositions of four antigenic variants of A/Memphis/1/71 (H3N2) influenza virus, which were selected by growth in the presence of monoclonal antibodies against the haemagglutinin, were compared. The results indicate that the mutant haemagglutinin genes can be differentiated by polyacrylamide gel electrophoresis of the double stranded RNA hybrids formed between virion RNA and transcripts isolated from infected cells.

引用

收藏

页码：245 / 248

页数：4

相关论文

共 10 条

[1] METHOD FOR ASSIGNING TEMPERATURE-SENSITIVE MUTATIONS OF INFLUENZA-VIRUSES TO INDIVIDUAL SEGMENTS OF GENOME [J].

ALMOND, JW ;

MCGEOCH, D ;

BARRY, RD .

VIROLOGY, 1977, 81 (01) :62-73

[2] ANTIGENIC DRIFT IN INFLUENZA A VIRUSES .1. SELECTION AND CHARACTERIZATION OF ANTIGENIC VARIANTS OF A-PR-8-34 [H0N1] INFLUENZA-VIRUS WITH MONOCLONAL ANTIBODIES [J].

GERHARD, W ;

WEBSTER, RG .

JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 148 (02) :383-392

[3]

Hay A J, 1977, Dev Biol Stand, V39, P15

[4] TEMPERATURE-SENSITIVE MUTANTS OF REOVIRUS .2. ANOMALOUS ELECTROPHORETIC MIGRATION OF CERTAIN HYBRID RNA MOLECULES COMPOSED OF MUTANT PLUS STRANDS AND WILD-TYPE MINUS STRANDS [J].

ITO, Y ;

JOKLIK, WK .

VIROLOGY, 1972, 50 (01) :202-+

[5]

KILBOURN.ED, 1969, B WORLD HEALTH ORGAN, V41, P643

[6]

LAVER WG, VIROLOGY

[7]

PALMER DF, 1975, DHEW IMMUNOLOGY SERI, P89

[8] MAPPING OF INFLUENZA-VIRUS GENOME .3. IDENTIFICATION OF GENES CODING FOR NUCLEOPROTEIN, MEMBRANE-PROTEIN, AND NONSTRUCTURAL PROTEIN [J].

RITCHEY, MB ;

PALESE, P ;

SCHULMAN, JL .

JOURNAL OF VIROLOGY, 1976, 20 (01) :307-313

[9]

SCHOLTISSEK C, 1976, VIROLOGY, V74, P332, DOI 10.1016/0042-6822(76)90340-8

[10] INDEPENDENT VARIATION IN NATURE OF HEMAGGLUTININ AND NEURAMINIDASE ANTIGENS OF INFLUENZA VIRUS - DISTINCTIVENESS OF HEMAGGLUTININ ANTIGEN OF HONG KONG/68 VIRUS [J].

SCHULMAN, JL ;

KILBOURNE, ED .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 63 (02) :326-+