USE OF ELECTROSPRAY MASS-SPECTROMETRY IN ENZYMATIC STUDIES ON ACYL CARRIER PROTEIN IMPLICATED IN FATTY-ACID BIOSYNTHESIS IN SACCHAROPOLYSPORA-ERYTHRAEA

被引:7
作者
BRIDGES, AM [1 ]
LEADLAY, PF [1 ]
REVILL, WP [1 ]
STAUNTON, J [1 ]
机构
[1] CAMBRIDGE CTR MOLEC RECOGNIT,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND
关键词
D O I
10.1039/c39910000778
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Two types of experiments are used to confirm that an acyl carrier protein (ACP) is involved in fatty acid biosynthesis using a cell-free extract of Saccharopolyspora erythraea: turnover using a chemically prepared acyl derivative of the ACP, and isolation of acylated intermediates by the action of the fatty acid synthase were detected by electrospray mass spectrometry.
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页码:778 / 779
页数:2
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