ADENYLYL CYCLASE ACTIVITY OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE IS NOT REGULATED BY GUANYL NUCLEOTIDES

被引：11

作者：

ENGELBERG, D

PORADOSU, E

SIMCHEN, G

LEVITZKI, A

机构：

[1] HEBREW UNIV JERUSALEM,INST LIFE SCI,DEPT BIOL CHEM,IL-91904 JERUSALEM,ISRAEL

[2] HEBREW UNIV JERUSALEM,INST LIFE SCI,DEPT GENET,IL-91904 JERUSALEM,ISRAEL

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

Adenylyl cyclase; Schizosaccharomyces pombe; Yeast;

D O I：

10.1016/0014-5793(90)80605-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14. © 1990.

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页码：413 / 418

页数：6

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