FUNCTIONAL CLONING OF BUD5, A CDC25-RELATED GENE FROM SACCHAROMYCES-CEREVISIAE THAT CAN SUPPRESS A DOMINANT-NEGATIVE RAS2 MUTANT

被引：94

作者：

POWERS, S ^{[1
]}

GONZALES, E ^{[1
]}

CHRISTENSEN, T ^{[1
]}

CUBERT, J ^{[1
]}

BROEK, D ^{[1
]}

机构：

[1] UNIV SO CALIF,SCH MED,DEPT BIOCHEM,LOS ANGELES,CA 90033

来源：

CELL | 1991年 / 65卷 / 07期

关键词：

D O I：

10.1016/0092-8674(91)90017-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.

引用

页码：1225 / 1231

页数：7

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