CHEMICAL SYNTHESIS OF RNA USING FAST OLIGONUCLEOTIDE DEPROTECTION CHEMISTRY

被引：28

作者：

VINAYAK, R ^{[1
]}

ANDERSON, P ^{[1
]}

MCCOLLUM, C ^{[1
]}

HAMPEL, A ^{[1
]}

机构：

[1] NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/20.6.1265

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The exocyclic amine protecting groups in oligonucleotide synthesis which require 8-16 hours at 55-degrees-C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytosine). Using these fast oligonucleotide deprotecting groups which require 2-3 hours at 55-degrees-C for complete deprotection, a new set of cyanoethyl phosphoramidite ribonucleoside monomers and supports has been developed. Ribozymes and substrate RNAs which were synthesized with these phosphoramidites were assayed and were found to have full catalytic (biological) activity.

引用

页码：1265 / 1269

页数：5

共 30 条

[1]

AGARWAL S, 1990, TETRAHEDRON LETT, P7541

[2]

ANDERSON P, 1991, IN PRESS NUCLEIC ACI

[3]

ANDRUS A, Patent No. 5047524

[4] STUDIES ON NUCLEOTIDE CHEMISTRY .15. SYNTHESIS OF OLIGODEOXYNUCLEOTIDES USING AMIDINE PROTECTED NUCLEOSIDES [J].