CHARACTERIZATION OF A BOVINE SYNOVIAL-FLUID LUBRICATING FACTOR .1. CHEMICAL, SURFACE-ACTIVITY AND LUBRICATING PROPERTIES

被引：171

作者：

JAY, GD ^{[1
]}

机构：

[1] SUNY STONY BROOK,DEPT PATHOL,STONY BROOK,NY 11794

来源：

CONNECTIVE TISSUE RESEARCH | 1992年 / 28卷 / 1-2期

关键词：

TRIBOLOGY; CARTILAGE; SYNOVIAL FLUID; LUBRICIN; REPULSIVE HYDRATION FORCE;

D O I：

10.3109/03008209209014228

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A lubricating glycoprotein (PSLF) with an apparent molecular weight of 280 kDa was purified from bovine synovial fluid by anion exchange, molecular sieve chromatography, and density gradient centrifugation. Lubrication was measured under boundary conditions as lowering of the coefficient of friction (μ between oscillating natural latex and polished glass. Lubricating ability was first observed at a concentration of 200 μg/ml and became maximal at 260 μg/ml. Hydrophobic interfacial tension measurements indicated that at the former concentration, monolayers of PSLF formed. Sugar digestions showed that lubricating ability depends upon the terminal galactose of the molecule. PSLF is similar if not identical to lubricin. It is proposed that a repulsive hydration force is the molecular mechanism for lubricating activity. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：71 / 88

页数：18

共 48 条

[1] PROCESSING OF ADENOVIRUS 2-INDUCED PROTEINS [J].