VERSATILE GENE CASSETTE PLASMIDS TO FACILITATE THE CONSTRUCTION OF GENERALIZED AND SPECIALIZED CLONING VECTORS

被引：19

作者：

MONGKOLSUK, S ^{[1
]}

VATTANAVIBOON, P ^{[1
]}

RABIBHADANA, S ^{[1
]}

KIATPAPAN, P ^{[1
]}

机构：

[1] CHULABHORN RES INST,BIOTECHNOL LAB,BANGKOK,THAILAND

来源：

GENE | 1993年 / 124卷 / 01期

关键词：

CHLORAMPHENICOL ACETYLTRANSFERASE; BETA-GLUCURONIDASE; TETRACYCLINE-RESISTANCE; MOBILIZING SEQUENCE;

D O I：

10.1016/0378-1119(93)90773-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have built a series of useful gene cassette plasmids to facilitate the construction of generalized and specialized cloning vectors. The gene cassettes consist of two promoter-less genes, cat and gus, a selectable marker gene (tet) and an IncP mob sequence. All of these genes, in the cassette vectors are flanked by many unique restriction sites to facilitate their use in the construction of cloning vectors.

引用

页码：131 / 132

页数：2

共 5 条

[1] CONSTRUCTION AND CHARACTERIZATION OF THE CHLORAMPHENICOL-RESISTANCE GENE CARTRIDGE - A NEW APPROACH TO THE TRANSCRIPTIONAL MAPPING OF EXTRACHROMOSOMAL ELEMENTS [J].