PROGRESSIVE SATURATION AND SATURATION-TRANSFER ESR FOR MEASURING EXCHANGE PROCESSES OF SPIN-LABELED LIPIDS AND PROTEINS IN MEMBRANES

被引：18

作者：

MARSH, D

机构：

[1] Max-Planck-Inst. F. Biophysik. Chem., Abteilung Spektroskopie, D-37077 Göttingen, Germany

[2] Spectroscopy Department, Max Planck Inst. Biophysical Chem., Göttingen, Germany

[3] University of Oxford

[4] Astbury Department of Biophysics, University of Leeds

[5] Biology Division, National Research Council of Canada, Ottawa, Ont., Canada

[6] Max Planck Institute, Göttingen, Germany

[7] Biochemistry Department, University of Oxford

来源：

CHEMICAL SOCIETY REVIEWS | 1993年 / 22卷 / 05期

关键词：

D O I：

10.1039/cs9932200329

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Continuous wave ESR spectroscopy of spin-labelled species is sensitive to molecular rate processes occurring on the timescale of the nitroxide spin-lattice relaxation, which lies in the microsecond regime. The experiments fall into two classes: either the progressive saturation of the conventional ESR spectrum, or recording of the saturation transfer ESR spectrum that is detected in phase quadrature under conditions of partial saturation. Rate processes that may be studied in this way are slow two-site exchange and weak Heisenberg spin exchange. Applications include those to the study of biological membranes.

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页码：329 / 335

页数：7

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