MONOCYTIC DIFFERENTIATION OF A MYELOMONOCYTIC LEUKEMIC-CELL (WEHI 3B JCS) IS INDUCED BY TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA)

被引:24
作者
MAK, NK
FUNG, MC
LEUNG, KN
HAPEL, AJ
机构
[1] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV CLIN SCI,EXPTL HAEMATOL GRP,POB 334,CANBERRA,ACT 2601,AUSTRALIA
[2] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,MED MOLEC BIOL GRP,CANBERRA,ACT 2601,AUSTRALIA
关键词
D O I
10.1006/cimm.1993.1173
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have isolated a subclone (JCS) of the WEHI 3B myelomonocytic leukemia, which acquires the characteristics of mature macrophage lineage cells in the presence of PMA or noncytotoxic concentrations of TNF-α(600-1200 U/ml). JCS cells were compared with D+ and D- subclones of WEHI 3B. Unlike D+ cells, JCS cells did not produce differentiated granulocyte-macrophage colonies in the presence of postendotoxin serum or recombinant G-CSF. Stimulation with PMA or TNF-α reduced proliferation of JCS cells. TNF-α decreased the level of cell surface J11D antigen with concurrent increased expression of Mac-1 and FcR antigens and phagocylic activity. These TNF-α-mediated effects were enhanced by addition of IFN-γ to the cultures. Furthermore, differentiation-inducing activity of PMA could be prevented using neutralizing anti-TNF-α antibodies. The results indicate that exogenous TNF-α can act as a differentiative agent for JCS cells and that endogenous TNF-α is the active substance when PMA is used to stimulate macrophage differentiation of JCS cells. © 1993 Academic Press. All rights reserved.
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页码:1 / 14
页数:14
相关论文
共 27 条
[1]  
BHALLA AK, 1991, IMMUNOLOGY, V72, P61
[2]   GRANULOCYTIC COLONY-STIMULATING FACTOR (G-CSF) DOES NOT INDUCE DIFFERENTIATION OF WEHI3B(D+) CELLS BUT IS REQUIRED FOR THE SURVIVAL OF THE MATURE PROGENY [J].
BOHMER, RM ;
BURGESS, AW .
INTERNATIONAL JOURNAL OF CANCER, 1988, 42 (01) :53-58
[3]  
CAUX C, 1991, BLOOD, V78, P635
[4]  
DEBENEDETTI F, 1990, BLOOD, V75, P626
[5]   PCR AMPLIFICATION OF MESSENGER-RNA DIRECTLY FROM A CRUDE CELL LYSATE PREPARED BY THERMOPHILIC PROTEASE DIGESTION [J].
FUNG, MC ;
FUNG, KYM .
NUCLEIC ACIDS RESEARCH, 1991, 19 (15) :4300-4300
[6]  
GOUGH NM, 1989, REPROD FERT DEVELOP, V1, P2281
[8]  
JAATTELA M, 1991, LAB INVEST, V64, P724
[9]  
JOHNSON CS, 1991, EXP HEMATOL, V19, P101
[10]  
KAMIJO R, 1990, J IMMUNOL, V144, P1311