A MODIFIED MOUSE PERIPHERAL-BLOOD LYMPHOCYTE CULTURE SYSTEM FOR CYTOGENETIC ANALYSIS

被引：47

作者：

EREXSON, GL

KLIGERMAN, AD

机构：

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1987年 / 10卷 / 04期

关键词：

D O I：

10.1002/em.2850100407

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A detailed methodology is presented for culturing mouse peripheral blood lymphocytes isolated on density gradients and stimulated to divide using either phytohemagglutinin, concanavalin A, or lipopolysaccharide. The techniques described yield more than sufficient numbers of mitotic cells for analyzing sister chromatid exchange, chromosome aberrations, and micronuclei following in vitro or in vivo exposure to chemicals or radiation.

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页码：377 / 386

页数：10

相关论文

共 21 条

[1] A METHOD FOR PREPARING CHROMOSOMES FROM PERIPHERAL-BLOOD OF THE MOUSE [J].

BARREN, PR ;

MORRIS, SM ;

SCHOL, H ;

BISHOP, JB .

MUTATION RESEARCH, 1982, 104 (1-3) :159-163

[2] DIAZIQUONE-INDUCED MICRONUCLEI IN CYTOCHALASIN B-BLOCKED MOUSE PERIPHERAL-BLOOD LYMPHOCYTES [J].

EREXSON, GL ;

KLIGERMAN, AD ;

ALLEN, JW .

MUTATION RESEARCH, 1987, 178 (01) :117-122

[3] ANALYSES OF SISTER-CHROMATID EXCHANGE AND CELL-CYCLE KINETICS IN MOUSE LYMPHOCYTE-T AND LYMPHOCYTE-B FROM PERIPHERAL-BLOOD CULTURES [J].

EREXSON, GL ;

WILMER, JL ;

KLIGERMAN, AD .

MUTATION RESEARCH, 1983, 109 (02) :271-281

[4] INDUCTION OF CYTOGENETIC DAMAGE IN RODENTS AFTER SHORT-TERM INHALATION OF BENZENE [J].

EREXSON, GL ;

WILMER, JL ;

STEINHAGEN, WH ;

KLIGERMAN, AD .

ENVIRONMENTAL MUTAGENESIS, 1986, 8 (01) :29-40

[5] SIMULTANEOUS ASSESSMENT OF ETHYL CARBAMATE-INDUCED SCES IN MURINE LYMPHOCYTES, BONE-MARROW AND ALVEOLAR MACROPHAGE CELLS [J].

GOON, D ;

CONNER, MK .

CARCINOGENESIS, 1984, 5 (03) :399-402

[6] FACTORS INVOLVED IN DIFFERENTIAL GIEMSA-STAINING OF SISTER CHROMATIDS [J].

GOTO, K ;

MAEDA, S ;

KANO, Y ;

SUGIYAMA, T .

CHROMOSOMA, 1978, 66 (04) :351-359

[7] SUPPRESSION OF IMMUNITY BY STRESS - EFFECT OF A GRADED SERIES OF STRESSORS ON LYMPHOCYTE STIMULATION IN THE RAT [J].

KELLER, SE ;

WEISS, JM ;

SCHLEIFER, SJ ;

MILLER, NE ;

STEIN, M .

SCIENCE, 1981, 213 (4514) :1397-1400

[8] GENOTOXICITY STUDIES OF BENZ[L]ACEANTHRYLENE [J].

KLIGERMAN, AD ;

MOORE, MM ;

EREXSON, GL ;

BROCK, KH ;

DOERR, CL ;

ALLEN, JW ;

NESNOW, S .

CANCER LETTERS, 1986, 31 (02) :123-131

[9] SISTER CHROMATID EXCHANGE ANALYSIS IN LUNG AND PERIPHERAL-BLOOD LYMPHOCYTES OF MICE EXPOSED TO METHYL ISOCYANATE BY INHALATION [J].

KLIGERMAN, AD ;

CAMPBELL, JA ;

EREXSON, GL ;

ALLEN, JW ;

SHELBY, MD .

ENVIRONMENTAL MUTAGENESIS, 1987, 9 (01) :29-36

[10] INDUCTION OF SISTER-CHROMATID EXCHANGE (SCE) AND CELL-CYCLE INHIBITION IN MOUSE PERIPHERAL-BLOOD LYMPHOCYTES-B EXPOSED TO MUTAGENIC CARCINOGENS INVIVO [J].

KLIGERMAN, AD ;

EREXSON, GL ;

WILMER, JL .

MUTATION RESEARCH, 1985, 157 (2-3) :181-187