ISOLATION OF PI 4.5 SOYBEAN TRYPSIN INHIBITOR BY ISOELECTRIC FOCUSING

被引：11

作者：

CATSIMPOOLAS, N

EKENSTAM, C

MEYER, EW

机构：

[1] Protein Research Laboratory, Central Soya-Chemurgy Division, Chicago

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 175卷 / 01期

关键词：

D O I：

10.1016/0005-2795(69)90147-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soybean trypsin inhibitor was isolated from commercial crude soybean trypsin inhibitor and soybean whey protein by isoelectric focusing in the region between pH 3 and pH 10. The inhibitor was isoelectrically focused at pH 4.5. Disc electrophoresis in 11% polyacrylamide gels and immunoelectrophoresis in agar gel, using anti-crude soybean trypsin inhibitor and anti-soybean whey protein sera, indicated that the isolated inhibitor was not contaminated by other proteins. © 1969.

引用

页码：76 / +

页数：1

共 12 条

[1] ARTIFACT PRODUCED IN DISC ELECTROPHORESIS BY AMMONIUM PERSULFATE
BREWER, JM
[J]. SCIENCE, 1967, 156 (3772) : 256 - &
[2] CATSIMPOOLAS N, IN PRESS
[3] DAVIS DJ, 1964, ANN NY ACAD SCI, V121, P256
[4] *METHODE PERMETTANT LETUDE CONJUGUEE DES PROPRIETES ELECTROPHORETIQUES ET IMMUNOCHIMIQUES DUN MELANGE DE PROTEINES - APPLICATION AU SERUM SANGUIN
GRABAR, P
WILLIAMS, CA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1953, 10 (01) : 193 - 194
[5] HAGLUND H, 1967, SCI TOOLS, V14, P18
[6] CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR .2. GENERAL PROPERTIES
KUNITZ, M
[J]. JOURNAL OF GENERAL PHYSIOLOGY, 1947, 30 (04) : 291 - 310
[7] SOYBEAN TRYPSIN INHIBITORS - ISOLATION, PURIFICATION AND PHYSICAL PROPERTIES
RACKIS, JJ
ANDERSON, RL
MANN, RK
SASAME, HA
SMITH, AK
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1962, 98 (03) : 471 - +
[8] CHROMATOGRAPHY OF SOYBEAN PROTEINS .1. FRACTIONATION OF WHEY PROTEINS ON DIETHYLAMINOETHYL-CELLULOSE
RACKIS, JJ
SASAME, HA
ANDERSON, RL
SMITH, AK
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1959, 81 (23) : 6265 - 6270
[9] UNE MICRO-METHODE DE LIMMUNO-ELECTROPHORESE
SCHEIDEGGER, JJ
[J]. INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1955, 7 (02): : 103 - 110
[10] SVENSSON H, 1962, ARCH BIOCHEM BIOPHYS, P132

← 1 2 →