THE ROLE OF A CONSERVED GUANOSINE RESIDUE IN THE HAMMERHEAD-TYPE RNA ENZYME

被引：37

作者：

ODAI, O

HIROAKI, H

SAKATA, T

TANAKA, T

UESUGI, S

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,1-6 YAMADAOKA,SUITA,OSAKA 565,JAPAN

[2] PROT ENGN RES INST,SUITA,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1990年 / 267卷 / 01期

关键词：

Conformation; Hammerhead structure: NMR; RNA enzyme; Synthetic oligoribonucleotide;

D O I：

10.1016/0014-5793(90)80311-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have designed a hammerhead-type RNA system which consists of three RNA fagments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation. © 1990.

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页码：150 / 152

页数：3

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