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ENZYME-LINKED IMMUNOSORBENT MICRO-ASSAY FOR QUANTIFICATION OF SPECIFIC ANTIBODIES TO COLLAGEN TYPE-I, TYPE-II, TYPE-III

被引：126
作者：
GOSSLAU, B
BARRACH, HJ
机构：
[1] Institut für Toxikologie und Embryopharmakologie, Freie Universität Berlin
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 29卷 / 01期
关键词：
D O I：
10.1016/0022-1759(79)90127-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme-linked immunosorbent assay (ELISA) is described for determination of antibodies to collagen type I, II, III in serum or other body fluids. Polystyrene microplates are coated with either collagen I, II or III, and samples of body fluids being tested are incubated in the plates. Antibodies which attach specifically to the collagen are detected with anti-IgG antibody conjugated with peroxidase. Bound peroxidase is quantitatively estimated by the colour reaction produced with the substrate 5-aminosalicyclic acid. © 1979.
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页码：71 / 77
页数：7
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