IDENTIFICATION OF GIBBERELLIN A9 METHYL-ESTER AS A NATURAL SUBSTANCE REGULATING FORMATION OF REPRODUCTIVE-ORGANS IN LYGODIUM-JAPONICUM

被引:75
作者
YAMANE, H [1 ]
TAKAHASHI, N [1 ]
TAKENO, K [1 ]
FURUYA, M [1 ]
机构
[1] UNIV TOKYO,FAC SCI,DEPT BOT,BUNKYO KU,TOKYO 113,JAPAN
关键词
Antheridiogen; Archegonium formation; Gibberellin; Lygodium;
D O I
10.1007/BF00388747
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Prothallia of Lygodium japonicum (Thunb.) Sw. were aseptically cultured under white light in a mineral solution. Solvent fractionation of the resultant culture medium and subsequent preparative thinlayer chromatography yielded a fraction that induced antheridium formation and inhibited archegonium formation. Combined gas chromatography-selected ion monitoring analysis of this fraction confirmed the presence of gibberellin A9 methyl ester (GA9-me) as an antheridiogen and an inhibitor of archegonium formation. Exogenously applied [3H]GA9 was rapidly converted to [3H]GA9-me in the prothallial tissue. Authentic GA9-me was active to 10-10M in antheridium formation and to 10-9M in the inhibition of archegonium formation. © 1979 Springer-Verlag.
引用
收藏
页码:251 / 256
页数:6
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