GAP JUNCTION DISTRIBUTION IS ALTERED BETWEEN CARDIAC MYOCYTES INFECTED WITH TRYPANOSOMA-CRUZI

被引:74
作者
DECARVALHO, ACC
TANOWITZ, HB
WITTNER, M
DERMIETZEL, R
ROY, C
HERTZBERG, EL
SPRAY, DC
机构
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT NEUROSCI, BRONX, NY 10461 USA
[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT ANAT & STRUCT BIOL, BRONX, NY 10461 USA
[3] FED UNIV RIO DE JANEIRO, CARLOS CHAGAS FILHO INST, EXCITABLE MEMBRANES LAB, RIO DE JANEIRO, BRAZIL
[4] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT PATHOL, BRONX, NY 10461 USA
[5] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT MED, BRONX, NY 10461 USA
关键词
HEART; CHAGAS DISEASE; CONNEXIN-43; PARASITE; CHAGASIC CARDIOMYOPATHY;
D O I
10.1161/01.RES.70.4.733
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Conduction disturbances frequently accompany both acute and chronic Chagas' disease. To explore the possibility that changes in gap junction distribution of abundance might play a role in these disturbances, we have investigated intercellular communication between rat neonatal cardiac myocytes in cultures infected with Trypanosoma cruzi. Contractile activity of infected cells was characterized by regional asynchrony within the culture as well as by irregular contraction patterns. Junctional conductance between infected cell pairs was found to be significantly lower than in uninfected cell pairs, and the rapidity and extent of intercellular transfer of the dye lucifer yellow was markedly reduced between infected cells. Immunocytochemical studies demonstrated that the parasitic infection significantly decreased connexin 43 expression at junctional membrane regions, correlating with the detected functional uncoupling. These findings of reduced gap junction abundance and function in trypanosome-infected cells may provide important insight into the pathogenesis of the cardiac arrhythmias that attend Chagas' disease.
引用
收藏
页码:733 / 742
页数:10
相关论文
共 52 条
  • [1] EXPERIMENTAL ELECTROPHYSIOLOGY AND ARRHYTHMOGENICITY - ANISOTROPY AND VENTRICULAR-TACHYCARDIA
    ALLESSIE, MA
    SCHALIJ, MJ
    KIRCHHOF, CJHJ
    BOERSMA, L
    HUYBERS, M
    HOLLEN, J
    [J]. EUROPEAN HEART JOURNAL, 1989, 10 : 2 - 8
  • [2] AMORIM DS, 1982, BRIT HEART J, V47, P11
  • [3] ANDRADE ZA, 1981, ARCH PATHOL LAB MED, V105, P460
  • [4] HISTOPATHOLOGY OF CONDUCTING TISSUE OF HEART IN CHAGAS MYOCARDITIS
    ANDRADE, ZA
    ANDRADE, SG
    OLIVEIRA, GB
    ALONSO, DR
    [J]. AMERICAN HEART JOURNAL, 1978, 95 (03) : 316 - 324
  • [5] STAGE-SPECIFIC SURFACE-ANTIGENS EXPRESSED DURING THE MORPHOGENESIS OF VERTEBRATE FORMS OF TRYPANOSOMA-CRUZI
    ANDREWS, NW
    HONG, KS
    ROBBINS, ES
    NUSSENZWEIG, V
    [J]. EXPERIMENTAL PARASITOLOGY, 1987, 64 (03) : 474 - 484
  • [6] EFFECTS OF CELLULAR UNCOUPLING ON CONDUCTION IN ANISOTROPIC CANINE VENTRICULAR MYOCARDIUM
    BALKE, CW
    LESH, MD
    SPEAR, JF
    KADISH, A
    LEVINE, JH
    MOORE, EN
    [J]. CIRCULATION RESEARCH, 1988, 63 (05) : 879 - 892
  • [7] BENNETT MVL, 1988, GAP JUNCTIONS, P287
  • [8] ANTISERA DIRECTED AGAINST CONNEXIN-43 PEPTIDES REACT WITH A 43-KD PROTEIN LOCALIZED TO GAP-JUNCTIONS IN MYOCARDIUM AND OTHER TISSUES
    BEYER, EC
    KISTLER, J
    PAUL, DL
    GOODENOUGH, DA
    [J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (02) : 595 - 605
  • [9] CONNEXIN43 - A PROTEIN FROM RAT-HEART HOMOLOGOUS TO A GAP JUNCTION PROTEIN FROM LIVER
    BEYER, EC
    PAUL, DL
    GOODENOUGH, DA
    [J]. JOURNAL OF CELL BIOLOGY, 1987, 105 (06) : 2621 - 2629
  • [10] HEART CELLS IN CULTURE - SIMPLE METHOD FOR INCREASING PROPORTION OF MYOBLASTS
    BLONDEL, B
    ROIJEN, I
    CHENEVAL, JP
    [J]. EXPERIENTIA, 1971, 27 (03): : 356 - &