SYNTHESIS AND LOCALIZATION OF SYMBIONIN, AND APHID ENDOSYMBIONT PROTEIN

被引：22

作者：

FUKATSU, T ^{[1
]}

ISHIKAWA, H ^{[1
]}

机构：

[1] UNIV TOKYO, FAC SCI, INST ZOOL, BUNKYO KU, TOKYO 113, JAPAN

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1992年 / 22卷 / 02期

关键词：

PEA APHID; ENDOSYMBIOSIS; SYMBIONIN; GROEL; CHAPERONIN; MICROAUTORADIOGRAPHY; IMMUNOHISTOCHEMISTRY;

D O I：

10.1016/0965-1748(92)90155-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In an effort to know the function of symbionin, and aphid endosymbiont-specific protein homologous to Escherichia coli GroEL protein, its synthesis and localization in the host insect were investigated. Microautoradiography of the tissue sections revealed that symbionin is synthesized and localized exclusively in the bacteriocyte. Immunohistochemistry with anti-symbionin antiserum suggested also that symbionin is present only in the bacteriocyte, especially concentrated in the endosymbiont. Immunohistochemistry of the semi-ultrathin tissue sections demonstrated that symbionin is localized in the endosymbiont and not detected in the cytoplasm of the bacteriocyte. These results indicated that symbionin is synthesized, localized, and functions in the endosymbiont, suggesting that it functions for the endosymbiont's own sake rather than for the host insect.

引用

页码：167 / +

页数：1

共 30 条

[1] ULTRASTRUCTURAL-LOCALIZATION OF ANTIGENIC SITES ON OSMIUM-FIXED TISSUES APPLYING THE PROTEIN A-GOLD TECHNIQUE
BENDAYAN, M
ZOLLINGER, M
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1983, 31 (01) : 101 - 109
[2] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS
BONNER, WM
LASKEY, RA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01): : 83 - 88
[3] Buchner P., 1965, ENDOSYMBIOSIS ANIMAL
[4] MITOCHONDRIAL HEAT-SHOCK PROTEIN HSP60 IS ESSENTIAL FOR ASSEMBLY OF PROTEINS IMPORTED INTO YEAST MITOCHONDRIA
CHENG, MY
HARTL, FU
MARTIN, J
POLLOCK, RA
KALOUSEK, F
NEUPERT, W
HALLBERG, EM
HALLBERG, RL
HORWICH, AL
[J]. NATURE, 1989, 337 (6208) : 620 - 625
[5] PROTEINS AS MOLECULAR CHAPERONES
ELLIS, J
[J]. NATURE, 1987, 328 (6129) : 378 - 379
[6] GROE HEAT-SHOCK PROTEINS PROMOTE ASSEMBLY OF FOREIGN PROKARYOTIC RIBULOSE BISPHOSPHATE CARBOXYLASE OLIGOMERS IN ESCHERICHIA-COLI
GOLOUBINOFF, P
GATENBY, AA
LORIMER, GH
[J]. NATURE, 1989, 337 (6202) : 44 - 47
[7] PURIFICATION AND PARTIAL CHARACTERIZATION OF SYMBIONIN, AN APHID ENDOSYMBIONT-SPECIFIC PROTEIN
HARA, E
ISHIKAWA, H
[J]. INSECT BIOCHEMISTRY, 1990, 20 (04): : 421 - 427
[8] HARA E, 1990, SYMBIOSIS, V8, P271
[9] HOMOLOGOUS PLANT AND BACTERIAL PROTEINS CHAPERONE OLIGOMERIC PROTEIN ASSEMBLY
HEMMINGSEN, SM
WOOLFORD, C
VANDERVIES, SM
TILLY, K
DENNIS, DT
GEORGOPOULOS, CP
HENDRIX, RW
ELLIS, RJ
[J]. NATURE, 1988, 333 (6171) : 330 - 334
[10] MAINTENANCE OF APHID CELLS AND INTRACELLULAR SYMBIOTES OF APHIDS IN-VITRO
HINDE, R
[J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 1971, 17 (03) : 333 - &

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