CHARACTERIZATION OF THE NONFUNCTIONAL FAS LIGAND OF GLD MICE

Mice homozygous for either the gld or lpr mutation develop autoimmune diseases and progressive lymphadenopathy, The lpr mutation is characterized by the absence of functional Fas, whereas gld mice exhibit an inactive fast due to a point mutation proximal to the extracellular C-terminus, The structural repercussions of this amino acid substitution remain unknown. Here we report that fast is expressed at similar levels on the surface of activated T lymphocytes from gld and wild-type mice, Using a polyclonal anti-fast antibody, indistinguishable amounts of a 40 kDa protein are detected in both gld and wild-type splenocytes, The molecular model of Fast, based on the known structure of TNF-alpha, predicts that the Phe --> Leu gld mutation is located at the protomer interface which is close to the FasR interaction site. We conclude that the gld mutation allows normal fast biosynthesis, surface expression and oligomerization, but induces structural alterations to the Fas binding region leading to the phenotypic changes observed.