Detection of ileal symbiont intracellularis in porcine faecal samples by polymerase chain reaction

被引：14

作者：

McCormick, BM ^{[1
]}

Hasse, D ^{[1
]}

Monckton, RP ^{[1
]}

机构：

[1] DEPT AGR,BENDIGO 3550,VIC,AUSTRALIA

来源：

VETERINARY MICROBIOLOGY | 1995年 / 47卷 / 3-4期

关键词：

ileal symbiont intracellularis (ISI); polymerase chain reaction (PCR); proliferative Enteritis (PE); pigs; faeces;

D O I：

10.1016/0378-1135(95)00118-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ileal Symbiont Intracellularis (ISI), the organism causing proliferative enteritis (PE) in pigs was detected in faeces by the application of polymerase chain reaction (PCR). The assay based on a 319 base pair DNA fragment was used on faecal and mucosal samples derived from pigs either affected or unaffected with PE. As few as 10(3) ISI could be detected in pig faeces spiked with ISI. No amplification product was detected in the faeces of unaffected pigs but faeces of confirmed clinical cases were positive. This method offers an accurate, sensitive, easy to perform alternative to monoclonal antibody tests or histological examination post-mortem for the presence of ISI in pig herds.

引用

页码：387 / 393

页数：7

共 7 条

[1] CLONED DNA PROBES SPECIFIC FOR THE INTRACELLULAR CAMPYLOBACTER-LIKE ORGANISM OF PORCINE PROLIFERATIVE ENTERITIS [J].